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EndoFree Plasmid Maxi Kit (10)

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商品名称 : EndoFree Plasmid Maxi Kit (10)   
货      号：12362
品      牌：Qiagen 凯杰 经销
货      期： 现货

规      格：
数量 + -
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北京百奥创新科技有限公司
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商品详情

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Features

  • Less than 0.1 EU/µg DNA achieved
  • Integrated endotoxin removal
  • High yields of high-copy plasmid DNA
  • LyseBlue for optimum lysis and maximum DNA yield

Product Details

EndoFree Plasmid Kits provide anion-exchange-based endotoxin-free plasmid DNA purification. QIAfilter Cartridges enable fast lysate clearing by filtration. The purified DNA exceeds the purity obtained by 2 x CsCl gradient centrifugation and is suitable for advanced transfection-grade applications. The EndoFree Plasmid Buffer Set can be used for preparations of 10 mega or 5 giga transfection-grade plasmid or cosmid DNA preparations.

Performance

The EndoFree Plasmid Kits integrate an efficient endotoxin removal step into the plasmid purification procedure — no additional extractions or affinity columns to remove lipopolysaccharides are required. Bacterial lysates are cleared by filtration with a QIAfilter Mega-Giga or Maxi Cartridge, and plasmid DNA is purified on gravity-flow QIAGEN-tips containing anion-exchange resin. Yields of up to 500 μg (maxi), 2.5 mg (mega), and 10 mg (giga) purified DNA are achieved from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). Purified DNA is endotoxin free (<0.1 EU/µg DNA).

For purification of low-copy plasmids and cosmids, the EndoFree Plasmid Mega Kit is a better choice than the EndoFree Giga Plasmid Kit, due to the large culture volumes required and the limited capacity of the QIAfilter Mega-Giga Cartridge.

EndoFree Plasmid Kits remove bacterial endotoxins which are released during the lysis step and influence transfection of DNA into primary cells and sensitive cultured cells. The endotoxin-free DNA obtained from the EndoFree Plasmid Kits is highly suited for reproducible and reliable results in transfection (see figures  Plasmid purification method versus transfection efficiency and  Plasmid purity versus transfection efficiency and tables "Endotoxin levels in plasmid preparations" and "EndoFree DNA yields high transfection efficiencies with primary cells"). QIAGEN ultrapure endotoxin-free DNA is also suitable for gene therapy research and other sensitive applications.

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